Mahalo iāʻoe no ka kipaʻana i ke'ano.com. ʻO ka polokalamu kele pūnaewele āu e hoʻohana nei i ke kākoʻo css. No nā hopena maikaʻi loa, makemake mākou e hoʻohana ana i ka hoʻohanaʻana i kahi pūnaewele hou (a iʻole ka hōʻoleʻana i keʻano hoʻohālikelike i ka Pūnaewele Pūnaewele). I ka manawa manawa e hōʻoiaʻiʻo ai i ke kākoʻoʻana, e hōʻike mākou i ka pūnaewele me keʻanoʻole.
ʻO ka hoʻokumuʻana o nā hiʻohiʻona holoholona o nā hoʻololi hoʻololi o Model (MC) kahi kumu koʻikoʻi no ke aʻoʻana i ka MC. He kanalimakumamāhā nā kime keʻokeʻo o Zealand keʻokeʻo i loko o ka hui sham-hana i hanaʻia,ʻo ka hui nui (ʻo ka hui pulupulu) a me ka hui pū). Ma ka pūʻulu NPE, ua hōʻikeʻia ka disc disc i keʻano o keʻano o ke kino antererotel Ua laweʻiaʻo NP mai ka L1 / 2 Oververtebral disc e ka syringe a iʻole i loko. Ka laweʻana i kahi hole i ka iwi subchondral. ʻO nā kaʻina hana hana a me nā hana hana i ka hui o ka pūpū a me nā hui sham-hana likeʻole e like me nā hui o ka NP i ka hui NP Maloko o iaʻu, ua waihoʻiaʻo kahi'āpanaʻeha i loko o ka lua, i ka huiʻana,ʻaʻohe mea i hoʻokomoʻia i loko o ka lua. Ma hope o ka hana, ua hanaʻiaʻo mri scanning a me molecular colilogular i hanaʻia. Ua loli ka māka ma ka pūʻulu npe, akāʻaʻohe mea hōʻailona hōʻailona'ē aʻe i ka hui nuiʻana ma ka hui Sham-hana a me ka hui pū me aʻu. Ua hōʻikeʻia ka nānāʻana i ka moʻoleloʻo Hertological i kēlā me kēia prissural proplittery ma ka pūnaewele implantation ma Il-4, Il-a i hoʻonuiʻia ma Il-4 a me IL-17 a ma Il-17 a ma If-a ua hoʻonuiʻiaʻo Il-17 a iʻole ma Il-17 a iʻole ma Il-17 a ma Il-17 a ma If-a ua hoʻonuiʻiaʻo Il-17 a i If-17 Hiki i ka hāʻawiʻana o NP i loko o ka iwi subchondral ke hana i kahi hiʻohiʻona holoholona o mc.
Hoʻololi nā hoʻololi modic (mc) i nā leona o nā mea i huliʻia a me nā iwi ipo iʻikeʻia e ka magnetic consonance i hōʻikeʻia (mri). He mea maʻamau lākou i nā poʻe pilikino me nā hōʻailona e pili ana i nā hōʻailona1. Ua hoʻonuiʻia nā haʻawina he nui i ka mea nui o MC ma muli o kāna hui me kaʻeha haʻahaʻa (LBP) 2,3. de roos et al.4 a me modic et al.5 Ke kū hoʻokahi i wehewehe mua i nāʻano hōʻailonaʻekolu o ka subchondal ʻO keʻano modic aʻu e loli ai he hyponisense ma t1-paona Hōʻike kēia leona i nā mea i hoʻopauʻia a me nā mea i hoʻopiliʻiaʻo Vascular Vascular Branch Ua hoʻololiʻo Modic Type II e hōʻike i ka hōʻailona kiʻekiʻe ma luna o nā T1W a me T2W mau'ōlelo. Ma kēiaʻano o ka lesion, hiki ke loaʻa ka lukuʻana i ka luku Hoʻololiʻo MoDIC TOW III i III i ka hōʻailona haʻahaʻa i loko o T1W a me T2W mau'ōlelo. Ua nānāʻia nā leʻa sclerotic i nā hopena i nānāʻia. Ua manaʻoʻiaʻo Mc i kahi maʻi pathological o ka spine a pili pili i nā maʻi degenerative he nui o ka spine7,8,9.
Ke noʻonoʻo nei i kaʻikepili i loaʻa, ua hāʻawiʻia he mau haʻawina i nāʻike kiko'ī i loko o ka Etiology a me Pathological Plances o MC. Albert et al. Ua manaʻoʻiaʻo MC e kumu paha e ka laʻana o ka hernation8. Hu et al. Ua nīnauʻo MC i ka Districe disc Degeneration10. Ua manaʻoʻo Kroc i ka manaʻo o ka "Strud disc Repupture,"ʻO nā moku'āina e alakaʻi ai i nā lekiō i ka microtears i ka hopena. Ma hope o ka hoʻokumuʻana o CLEFT i hoʻokumuʻia, ka lukuʻana i ka lukuʻia e ka nucleus pulplass Ma e like al al. Shared kahi hiʻohiʻona like a hōʻikeʻia i kahi o ka autummummunity e hoʻopili ai i kahi kī nui i ke kī nui i loko o ka Pathogenesis o MC12.
ʻO nā'ōnaehana o nā'ōnaehana o keʻano he cd4 + t cd4 + t coltper lymphocytes, e pāʻani i kahi hana koʻikoʻi ma ka pathogenesis o autohgmunity13. Hōʻike houʻia ka subset subset thond i hana i ka sintkune sytokinel cytokine il-17, a hoʻoulu i nā culokine i nā organs e hana ai i ka mea i hoʻopauʻia ma ka improx E pāʻani pūʻo TH2 i kahi hana kūʻokoʻa i loko o ka Pathogenesis o nā pane o ka maʻi. Hōʻike o ka il-4 e like me kahi meaʻelele hiki ke alakaʻi i ka hopena o nā hopena immunopaological15.
ʻOiai he nui nā haʻawina e hoʻomaʻamaʻaʻia ma MC16,17,18,19,19,2,2,2,22,24,24,24,24,24,24,24,24,24,24 Hoʻohanaʻia e noiʻi i ka Etiology a iʻole nā hoʻomaʻamaʻa hou e like me ka mea i manaʻoʻia e like me ka mālamaʻana. I kēia manawa, he mau holoholona holoholona'ē aʻe wale nō o MC ua hōʻikeʻia e aʻo i nā mea hana ma lalo o nā mīkini pathological.
Kuhiʻia ma ka Pūnaewele AutoImMune i manaʻoʻia e Albert a me Ma, ua hoʻokumu kēia noiʻi i kahi pā uila a me ka hoʻopiliʻana i ka papa o ka pā o ka papa. ʻO nā pahuhopu'ē aʻe e nānā i nā hiʻohiʻona hiʻona o nāʻano holoholona I kēia hopena, ke hoʻohanaʻana i kā mākou papa hana me ka molebular biology, mri, a me nā haʻawinaʻo Mystolological e aʻo ai i ka holomua o M.
Ua make elua namela Miha ma ka hana o ka hana, a make ae nei ua make na Rabbits i ka manawa o Mri. ʻO ke koena 48 i laweʻia a hōʻikeʻia a hōʻikeʻole i nā hōʻailona maʻamau a iʻole neurological a iʻole nā hōʻailona neurological ma hope o ka hanaʻana.
Hōʻikeʻo MRI i ka ikaika o ka hōʻailona o kaʻili o ka hoʻopiliʻana i nā'āpana likeʻole ma nā lua likeʻole. Ua hōʻikeʻia ka ikaika o ka hōʻailona o ka M5 Vertebal i ka hui NPE ma waena o 12, 16 mau makahiki ma hope o ka hoʻokomoʻana i ka hōʻailona haʻahaʻa) (F2W o nā huiʻeluaʻelua o nā'āpana i hoʻopiliʻia ma keʻano like me ka manawa like (Fig. 1a, b).
(A) Lunamakaainana Sequentical Mris of the Rabit Lumbit Lumbar spine ma 3 mau manawa manawa. ʻAʻole i loaʻa nā inoa inoa inoaʻole i nā kiʻi o ka hui o ka sham-hana. (B)ʻO nā hiʻohiʻona hōʻailona o ke kino o ke kino vertebral ma keʻano o ka hui me nā hui e like me nā hui o ka sham-hana nui i ka pūnaewele sham-hana nui i ka pūnaewele i kauʻia ma luna o ka manawa nui. (C) i loko o ka hui npe,ʻikeʻia ka hōʻailona haʻahaʻa haʻahaʻa ma ka t1w sequence, aʻikeʻia ka hōʻailona hōʻailona a me ka hōʻailona haʻahaʻa Mai ka manawa 12-pule i ka manawa 20-pule, nā hōʻailona kiʻekiʻe e hoʻopuni ana i nā hōʻailona haʻahaʻa ma ka T2W Signels haʻahaʻa.
Hiki keʻikeʻia ka iwiʻo Hyperplasia ma keʻano o ke kino o ke kino o ka vertebral ma ka papa NPERBALL kino; Sham hui a me ka hui (First. 2c) 2a, b).
(A) ka papa o ke kino vertebral ma ka hapa nui, he nui ka lua, a he hyperlalo i ke kino vertebal. (B) keʻano o keʻano o ka pūnaewele i hoʻopiliʻia ma loko o keʻano o ka huiʻana i ka huiʻana o ka hana o ka hanaʻana i ka pūnaewele i hoʻonuiʻia ma ka manawa i hoʻopiʻiʻia ma ka manawa. ) Ua hoʻonui pinepineʻia ka iwi hyperlasia e hoʻonui a hoʻonuiʻia ma o ka disc intervertebral disc i ke kino o ka corralateral.
Hāʻawi i ka loiloi moʻomeheu e hāʻawi hou i nāʻike kiko'ī e pili ana i ka hoʻokumuʻana o ka iwi. Hōʻike ka kiʻi 3 i nā kiʻi o nā'āpana postoperative i hoʻopaʻaʻia me H & e. I ka hui pūʻana o ka hanaʻana, ua hoʻonohonoho maikaʻiʻia nā chondrindtes aʻaʻole iʻikeʻia ka protect ʻO ke kūlana ma o ka hui pūʻana me ia ma kēlā me kēia pūʻulu sham-hana (Fig. 3b). Eia nō naʻe, ma ka pūʻulu NP, kahi helu nui o nā chondrocytes a me ka hoʻohālike o nā np-like me nā papa np-like i nānāʻia ma ka pūnaewele implantation (Fig. 3c); 3c); 3c); 3c); 3c); 3c); 3c); 3c); 3c); 3c); 3c); 3c); 3c); 3c); 3c); 3c); 3c); 3c); 3c); 3c);
(A) hiki keʻikeʻia ke trabeculae kokoke i ka pā hope, ua paʻa nā chondrocytes me ka nui o ke kelepona a me keʻano. (B) ke kūlana o ka pūnaewele implantation ma keʻano o ka hui e like me nā pūʻulu sham. Hiki keʻikeʻia nā trabeculae a me nā chondrocytes, akāʻaʻohe mea maikaʻi loa i ka pomliliration ma ka pūnaewele implantation (40 mau manawa). )
ʻO ka hōʻike o interlukun 4 (IL-4) Mrna, Interlelequuin 17 (IL-(Il-) a me nā hui. I ka wā i hoʻohālikelikeʻia ai nā kiʻekiʻe o nā hua'ōlelo o nā hua'ōlelo (P <0.05). Ua hoʻohālikelikeʻia me ka hui hana sham hana, nā pae hōʻike o ka il-4, il-17, a me Ifn-γ
ʻO ka MRNA i hōʻikeʻia o ka IL-4, IL-17 a ma IFN-γ
I ka hoʻohālikelikeʻana,ʻaʻole i hōʻikeʻia nā pae hōʻike i loko o ka hui me kaʻokoʻa me keʻano heʻokoʻa (P> 0.05).
Ua hanaʻia ka nānāʻana o ke Komohana Komohana i ka hoʻohanaʻana i nā mea kūʻai aku i loaʻa i nā antiboby i loaʻa i ka il-4 a me Il-17 e hōʻoia ai i ka hōʻike MRNA i hoʻololiʻia. E like me ka hōʻikeʻana i nā kiʻi 5A, B, i hoʻohālikelikeʻia me Me Me ka hui pūʻana a me ka Spomment Group Ge Il-4 lāuaʻo Il-15 ma ka pūʻulu Hoʻokomoʻia me ka punawai hana shum hana, nā pae palekana o ka il-4 a me Il-17 ma kahi o nā loli nui.
) (B) blot o ke komohana.
Ma muli o ka palena palena o nāʻano hana i loaʻa i ka wā o ka hanaʻana, e hoʻomaʻemaʻe a me nā kiʻi kiko'ī ma luna o ka pathogenesis o MC he mea paʻakikī. Ua ho'āʻo mākou e hoʻokumu i kahiʻano holoholona holoholona o mc e aʻo i kona mau papa hana pathological prosot. I ka manawa like,ʻo ka loiloi loiloi:ʻo ka loiloi moʻolelo a me ka loiloi molecular a me molecular a me ka molecular cuilogical i hoʻohanaʻia e hahai i keʻano o mc i hoʻopiliʻia e np autograft. Ma muli o ka hopena, keʻano o ka hoʻohālikelikeʻana o NP i kahi loli lōʻihi ma ka helu hōʻailona ma ka hola 12 Ua hōʻoia i nā loiloi loiloi i hōʻoia i nā hopena o ka noiʻi radiological.
Ua hōʻikeʻia nā hopena o kēia hoʻokolohua a hiki mai nā loli a me nā mea hoʻololi i hiki mai ma ke kahua o ke kino o ka manuʻole o ka lehulehu. I ka manawa like,ʻo ka hōʻike o ka il-4, il-17 a me Ifn-γ Hiki i ke kino ke kumu i kahiʻano hōʻailona hōʻailona a me nā loli morphological. He mea maʻalahi ke loaʻa i nā hiʻohiʻona hōʻailona o nā kino o nā holoholona o nā holoholona holoholona. E hōʻoia i ka nānāʻana o nā mea nānā a me ka gross hetomy,ʻo ia nō,ʻo ia hoʻi,ʻo nā loli i ka holomua o nā cells kino. ʻOiai keʻano o ka paneʻana o ka maʻi ma muli o ka maʻi trauma ma hope o nā puncture, i hōʻikeʻia e ka nui o nā hōʻailona a me nā loli o nā hoʻololiʻana o MR. Hōʻike kēia mau hopena i nāʻano o ka autologry veltebral np heʻano kūpono no ka hoʻokumuʻana i ka holomua o ka holomua.
Pono kēia kumu hoʻohālike i ka mākaukau kūpono, manawa, a me ka hoʻoikaikaʻana. I nā hoʻokolohua premiliinary, ka hanaʻole a iʻole ka hoʻouluʻana a iʻole ka hoʻouluʻana o nā'ōnaehana mika o ka paraverticaral ligamentous. Pono e mālamaʻia e hōʻino a hōʻino paha i nā discs adjan. Mai ka neʻeʻana o ka hohonu o ka hanaʻana e kiʻiʻia e loaʻa i nā hopena kūpono a me nā hopena hou, hana maʻamau mākou i kahi plug eʻoki ana i keʻano o kahi mea he 3 mm lōʻihi lōʻihi. Ka hoʻohanaʻana i kēia plug e hōʻoia i ka hohonu o ka hohonu o ka hohonu o ke kino vertebal. Ma nā hoʻokolohua mua,ʻekolu mau mea hana o Orthopedia i pili i ka hana i loaʻa i nā hana he 16-Gauge pono e hana me nā hana 18-Gauge. No ka haʻaleleʻana i ka waiʻumi nui i ka wā o ka laweʻana, e paʻa ana i ka pono no kahi manawa e hāʻawi ai i kahiʻano kūpono e hiki ai ke kāohiʻia i kēiaʻano.
ʻOiai he nui nā haʻawina he nui i hoʻopaʻaʻia i ka mc, liʻiliʻi liʻiliʻi e pili ana i ka Etiology a me Pathogenesis o MC25,26,27. E pili ana i kā mākou mau noiʻi mua,ʻike mākou e pāʻani ana ka autoommunity i kahi kī nui i keʻano a me ka hoʻomohalaʻana o MC12. Ua nānā kēia noiʻi i ka hōʻike nui o ka il-4, Il-17, aʻo Ifn-γ I kā mākou noiʻiʻana, Hoʻohālikelikeʻia me ka hui maikaʻiʻole, ua kiʻekiʻe ka pūʻulu nike {4, a i Il-17, aʻo Il-ʻekolu.
Hoʻoikaikaʻia, il-17 mrna i ka hōʻike i nā np cell mai nā maʻi maʻi me ka hernation discation28. Ua hoʻonuiʻia nā IL-4 a me Ifn-γ Il-17 E hoʻokani i kahi kī nui ma ka maʻi inflammation, nā hōʻehaʻeha i ka maʻi autoimmune e pane a hoʻonui i ka paneʻana i ka Ifmune i Ifn-γ31. Ua hōʻikeʻia kaʻehaʻeha i ka maʻi Il-17 Il-4 Hiki i ka hōʻikeʻana i ka hōʻike o nā cytkines cintxhemmatory (e like me ka IL-1 a me TNTURGE). Ua hōʻikeʻiaʻo ia ka hōʻailona o ka hōʻike o ka IL-4 iʻokoʻa i ka pūʻulu NPA i hoʻohālikelikeʻia i Il-17 a ma Ifn-γ i ka manawa like; Uaʻoi aku ka nui o ka MRNA i ka ISN-γ i loko o ka hui NP ma mua o kēlā mau hui'ē aʻe. No laila,ʻo Ifn-γ form e lilo i mea hoʻolaha e pili ana i ka paneʻana o ka pane o ka maʻi maʻi e hoʻopiliʻia e NP Intercation. Ua hōʻikeʻia nā haʻawina ma Ifn-γ
Hōʻikeʻia kēia noiʻi e pili ana i ka pane autoummune e pili ana i ka hanana a me ka hoʻomohalaʻana o mc. Luoma et al. ua loaʻa e like me nā hiʻohiʻona hōʻailona o mc a me nā mea kaulana np e like me mri, a hōʻike iʻelua hōʻailona hōʻailona ma T2W kiʻekiʻe ma T2W Standard38. Ua hōʻoiaʻia kekahi mau cytokines e pili kokoke i ka hopena o ka mc, e like me Il-139. Ma e like al al. Ua manaʻoʻia e piʻi i luna a i lalo paha i lalo o ka np paha he mana nui paha ma ka hanana a me ka hoʻomohalaʻana o MC12. Ua hōʻikeʻo Bobchko40 a me Herzbein et al.41 i hōʻikeʻiaʻo NP he immunotolerantolerantolerantolerantolerantle hikiʻole ke komo i ka wā hānau mai ka wā hānau. Ua hōʻike akuʻo NP Prottrouss i nā kino o ke kai i loko o ka hāʻawiʻana o ke koko, ma laila me ka hoʻolahaʻana me ka hoʻolahaʻana i ka OutoimMune Lotoummune Rouptions42. Hiki i nā hopena o Autorimmune ke hoʻonāukiuki i kahi nui o nā mea nui, a i ka manawa e hoʻomau mauʻia kēia mau mea i nāʻili, hiki iā lākou ke hana i nā loli i ke kau inoa inoa. Ma kēia noiʻi, Overrexpression o Il-4, Il-17 a me Ifn-γ a me Ifn-γ ʻO kēiaʻano holoholona holoholona maikaʻi loa i ka breatould a komo i loko o ka papa hope. Ua hōʻike houʻia kēia kaʻina hana i ka hopena o ka automimmunity ma mc.
E like me ka mea i manaʻoʻia, hāʻawiʻia kēia kumu holoholona hihiu iā mākou me kahi papa hana hiki ke aʻo i ka MC. Eia nō naʻe, loaʻa i kēia mau hiʻohiʻona he mau palena: mua, i ka wā o keʻano o ka nānāʻana i nā holoholona. ʻO ka lua,ʻoiai heʻekolu mau manawa e kauʻia ma kēia aʻoʻana,ʻaʻole naʻe mākou i hoʻololi i keʻano o ke kaʻina hana o ke kanaka. ʻoi aku ka maikaʻi o ka nānāʻana i nā loli hōʻailona. Ekolu No ka lano, ua hoʻohanaʻia ka microscoppy māmā māmā e hoʻopaʻa i ka hoʻokumuʻana o FUBROCARTLAGARTLAGARTLAGE i ka TRBBITEBERSEBRALST55. ʻO nā hopena lōʻihi loa o NP ma MC ma MC a me ka hoʻihoʻi hou i ke aʻo houʻana.
He kanalimakumamāleʻehā kāne kāneʻo New Zealand i ka nui o nā keiki liʻiliʻi e pili ana iā 2.5-3-3 Ua aponoia na hana kumuhana loa e Ana me na Ahatika o Tianjin isakona, a me nā ala hoʻokolohua i like me ke kuhikuhi o ka hoʻokolokolo.
Ua hanaʻia kekahi mau hoʻomaikaʻi i keʻano o keʻano hana o S. SABAjima 46. Ua kauʻia kēlā me kēia rabbit i kahi kūlana resumbency a me ka papa o ka anterior o nā disc overtebral lumberal i hōʻikeʻia i kahi kiʻi retroperiteral retroperiteral retroperitonel Hāʻawiʻia kēlā me kēia rabbit ma kahi o ka anesthesia maʻamau (20% urethane, 5 ml / kg ma ka vein viin). Ua hanaʻia kahi uluʻana o kaʻili o kaʻili i lalo mai nā'āpana o nā iwi i ka brim pelvic, 2 cm ventral. ʻO ka spine anterolallal kūpono mai ka L1 i L1 i ka L6 i hōʻikeʻia e SUMP a me ka palau o ka nui o ka subcutaneous, a me nā musperticonel resroritone. Ua hoʻoholoʻia ka pae disc e hoʻohana ana i ka brim pelvic ma keʻano he'āinaʻo Anatomical no ka pae disc disc disc pae. E hoʻohana i kahi pākeke pākuʻu 16-Gauge e huki i kahi lua ma kahi o ka pā o ka L5 vertebra i kahi hohonu o 3 mm (Fig. 6b). E hoʻohana i kahi syringe 5-ml e like me ka mea e like me ka punches nucleus nucleus plptsus ma ka disc (First. 6c). E wehe i ka plusus plucleus a iʻole kaʻiʻo e like me nā koi o kēlā me kēia hui. Ma hope o ka neʻeʻana o ka luawai Drill
(A) i ka l5-l6 disc disc i hōʻikeʻia ma o kahi kiʻi retroperitonel retroperitonel (B) E hoʻohana i kahi pono 16-Gauge e hoʻoheheʻe i kahi lua kokoke i ka L5 indplate. (C) nā mfs otoarous mfs.
Ua lawelaweʻiaʻo General Ansthesia me 20% Urethane (5 ML / KG) ma ka Vini Wind Vein ma 12, a me 20 mau wiki
Ua kaumahaʻia nā poʻe makemake e ka intramuscular i loko o ketamine (25.0 mg / kg) a me 20 mau pule Ua laweʻia ka spine holoʻokoʻa no ka nānāʻana i ka hōʻike a me ka loiloi maoli. ʻO ka inoa inoa inoa inoa hou (RT-QPCR) a me keʻano o ke komohana i hoʻohanaʻia eʻike i nā loli i nā mea nui i nā mea nui.
Hanaʻia nā hōʻike MRI ma nā papa inoa o ka hoʻohanaʻana i kahi papa lapaʻau 3.0 T Magnet. ʻO nā mea nui a nā mea i hanaʻia me 20% urethane (5 ml / kg) ma keʻano he nui o ka pepeiao Ua loaʻa iā CODOLOL T2-mau kiʻi i ke kālā (TR, 1445 ms Ua loaʻa iā Saigitttal Plane T2-mau paona i loaʻa me nā hoʻonohonoho e pili ana i kēia manawa Visual Curt of 260 a meʻewalu stimuli; ʻO ka mānoanoaʻokiʻia he 2 mm,ʻo ka gap ka 0.2 mm.
Ma hope o ka laweʻiaʻana o ka kiʻi hope loa a ua pepehiʻia ka poʻe hope hope loa,ʻo ka shamle, muscle-kauʻia, a ua laweʻia nā disc disc. Ua hoʻopaʻaʻia nā Tissues i 10% Natural Buiffed Stated form no 1 pule, e hoʻokaʻawaleʻia me kaʻaoʻao o ka cedicediacetracetic. Ua hoʻopiliʻia nā poloka o nā pahu i ka paraffin aʻokiʻoki i loko o nā'āpana sagittl (5 μm) me ka hoʻohanaʻana i kahi microtome. Ua kiʻiʻia nā'āpana me ka hematoxylin a me Eosin (H & e).
Ma hope o ka hōʻiliʻiliʻana i nā diss inververtebral mai nā kāpiliʻana i kēlā me kēia hui, ua laweʻiaʻo RNA i kahi'ōlelo a ACTUNDIRD , Madison, WI, USA). Ua hanaʻia ke kākau inoa inoa.
Ua hanaʻo RT-QPCR i ka hoʻohanaʻana i kahi prism 7300 (pili i ka colissystems staymix ʻO ka nui o ka helu PCR i ka 20 μL a ua loaʻa iā 1.5 μm μl o Dilited CDNA a me 0.2 μm o kēlā me kēia mua. Ua hoʻolālāʻia nā mea mua e ka mea hoʻolālā Oligoperfefect (Invitrogen, Valencia, Ca) Ua hoʻohanaʻia nā kūlana o nā kūlana TRANMA ma mua Ua hanaʻia nā anaʻana no 1 min ma 72 ° C. Ua hoʻopiliʻia nā hiʻohiʻona a pau iʻekolu mau manawa a ua hoʻohanaʻia ka waiwai a me ka waiwai maʻamau no ka loiloi RT-QPCR. Ua nānāʻia nāʻikepili amplication i ka hoʻohanaʻana i ka flexstation 3 (Molecular mau mea hana, Sunnyvale, CA, USA). Il-4, Il-17, a me Ifn-γ Ua heluʻia nā kiʻekiʻe o ka hōʻike o ka mea i hōʻikeʻiaʻo Mrna Mrna i keʻano 2-δδδ6.
Ua laweʻia ka papa hana o ka protein mai ka hoʻohanaʻana i kahi homogerali limogerali Ua hoʻoukaʻia nā kekelē kanalima o ka protein ma kēlā me kēia lane, i hoʻokaʻawaleʻia e 10% seds-ʻaoʻao, a laila laweʻia i kahi membrane pvdf. Ua hanaʻia ka block i ka 5% nonfat maloʻo maloʻo ma Tris-Bilfred Salline (TBS) i loaʻa 0.1%ʻumikūmālua 20 no 1 H i ka mahana o ka lumi. Ua hōʻikeʻia ka membrane me Rabbit anti-Decorin Pricay Antibody antibody (DILTED 1: 200 Me ka lua o ka antibody anti-goat anti-rabbit immunglobulin g at 1: 40,000 diol) i hui pūʻia me ka mahana o ka mahana. Uaʻikeʻia nā hōʻailonaʻo Western Blo No ka loiloi Densitometric, ua hoʻopaʻaʻia nā blots a me ka heluʻana i ka polokalamu uila a me nā hopena o ka waihona o ka Thanmuroactivity.
Ua hanaʻia nā helu helu statistical i ka hoʻohanaʻana i ka helu SPSS 50. SPSS, USA). Ua hōʻiliʻiliʻia nāʻikepili i ka wā o ka hoʻopaʻaʻana i ke aʻoʻana ma keʻano heʻano kūlike o keʻano o keʻano o nāʻano likeʻole ma waena o nā huiʻelua. Ua noʻonoʻoʻiaʻo P <0.05 i kahi koʻikoʻi statistically.
No laila,ʻo ka hoʻokumuʻana o kahiʻano holoholona holoholona o mc ma ka hoʻopiliʻana i nā npspors a me ka hanaʻana i ka mea hana macrolictatical nā poʻe pāʻani.
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POST manawa: Dec-13-2024