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ʻO ka uluʻana o ka microbial i nā hōʻeha pinepine iā ia iho e like me biofilms ʻO nā mea kūʻai kālā kālā hou e hoʻopili ai i nā maʻi hōʻeha hōʻeha, akāʻaʻole iʻike pinepineʻia kā lākou hopena maikaʻi loa. Ke hoʻohana nei ma Vitro a me Vivo Bifilm mau hiʻohiʻona o Staphylococcus a me Psedomontas Aeriginos Aeriginos ʻO nā lole AG1 + i loaʻa i ka clridetraceteratic acid and benzethonium chloride (AG1 + / EDTA / RC) ,ʻo wai ka hana a AG1 + AG2 + a me AG3 +O e hoʻokūkū i BioFilm a me kona hopena e ho'ōla ai. Ua loaʻa i nā hanana AG1 + mau mea liʻiliʻi i ka hopena i ka biofilm ma vitro a me nā mice (c5jl / 6j). Ma ka hoʻohālikelikeʻana, OXXYGENT AG BROTS a me AG1 + / EDTA / BC i ka nui o nā biofilms i loko o nā biofilms. Ua hopena nā lounāwai ma nā hale o nā mea momonaʻo Biolimm-insedlelo Biofilm-insifelm-insizelm-insiglom ʻO nā mea hoʻonaninani likeʻole o nā lole kālā
Ua weheweheʻia nā mea maʻi e like me nā "hōʻeha e hikiʻole ke holomua ma o nā hana maʻamau o ka ho'ōlaʻana i kahiʻano kūpono a me keʻano". E hana i nā mea hōʻeha i kahi noʻonoʻo, ka wahine a me ke kaumaha a me ka hoʻokele waiwai no nā maʻi a me ka'ōnaehana olakino. ʻO nā mea hana makahiki makahiki ma mua o ka mālamaʻana i nā hōʻeha a me nā mea e pili ana i nā coupbicties e pili ana i ka £ 8.3 biliona ma 2017-182. ʻO ka pilikia o nā mea kanu i kēia manawa maʻAmelika Hui PūʻIa, me Medicare e koho ana i ka uku makahiki o ka mālamaʻana i nā mea maʻi ma $ 28.1 billion3.
ʻO ka maʻi he mea nui e pale ai i ka hōʻehaʻana i ka hōʻehaʻana. ʻO nā maʻi pinepine i hōʻike pinepineʻia e like me Biofilms,ʻo ia kahi i kēia manawa ma 78% o nā hōʻeha non-resroning. ʻO Biofilms Biofilms i ka wā e hoʻopiliʻia ai nā microorganisms i nā papa, e like me nā papa hōʻeha Hoʻopiliʻia ka biofilm biofilm me ka hoʻonuiʻana i ka pane o ka maʻi e alakaʻi ana i ka pōʻino i ka pōʻino, hiki ke hoʻopau a pale paha i ka ho'ōlaʻana. ʻO ka hoʻonuiʻana i ka hōʻehaʻana i ka pōʻino paha ma muli o ka mahele i ka hoʻonuiʻana o nā metrix Metrix Eia kekahi, nā wahi i nā mea i hanaʻia a me nā biofilms i nā mea kūʻai aku he nui o ka oxygen a hiki ke kumu i ka nui o ka vital oxygen
ʻO nā biofilms nui loa i nā mea antiminchenia antimicrobial, e koi ana i nā hoʻolālā ikaika e mālama ai i ka maʻi biofilm No ka mea hiki i nā biofilms ke hoʻoponopono hou i nā antimirate, hiki i nā antimichubial e hōʻemi i ka pilikia o ka hana houʻana ma hope o ka hanaʻana i ka mea hana
Hoʻohana pinepineʻia ke kālā ma nā lole antimicrobial a ua hoʻohana pinepineʻia e like me ka mālama muaʻana no ka hōʻehaʻana i nāʻeha. Nui nā lole kālā kālepa i loaʻa i nā mea kūʻai kālā i loaʻa i kēlā me kēia mea i loaʻa i kahiʻano kala kala'ē aʻe, ka neʻeʻana, a me ka pākēneka. Ua alakaʻiʻia nā holomua o nā kālā kālā i ke kūkuluʻana i nā Armband kālā hou. ʻO keʻano metallic o ke kālā (AT0) E hoʻokō i ka hopena antiimicrobial Loaʻa nā lole kālā kālā i loaʻa i nā kālā kālā a iʻole nā mea kālā paha, i ka wā e hōʻikeʻia ai i ka wai, e hōʻikeʻia e hana ai i ka hana ag1 + ions. ʻO kēia mau makahikiʻo Ag1 + e hana me ka cell bacterial, e wehe ana i nā electrons mai nā kaʻina hana a iʻole nā kaʻina hana koʻikoʻi no ke olaʻana. Ua alakaʻiʻo Pasted i kaʻenehana i ke kūkuluʻana i kahi hui kālā kālā hou, agxysts (kālā nitrate, Ag7no11) ʻAʻole i koho i ke kālā maʻamau,ʻo ka decomposition o ka oxygen-nā mea momona e hana ai i nā moku he nui me nā valena kiʻekiʻe (ag3 +). Ma nā haʻawina Vitro ua hōʻikeʻia he mau haʻahaʻa haʻahaʻa haʻahaʻa o nā salts iʻoi aku ma mua o ka belosucisa, Staphylococus adiuchinia Auscoucolis asigial ʻO kekahiʻano kiʻi kiʻi hou e pili ana i nā mea kūʻai aku e pili ana i nā mea kanu hou,ʻo ia ka inoa o ka chlofiltetracetic. ʻO kēia mauʻenehana kālā kālā hou e hāʻawi i nā ala hou e hoʻopaʻa i nā biofilms. Eia nō naʻe,ʻo ka hopena o kēia mau antimicrobial i ka hōʻeha a me ka ho'ōlaʻana i ka nohona maikaʻiʻole e hōʻoia i ka hopena kūponoʻole a hoʻopauʻole i kahi hoʻomaha. Ua hōʻikeʻia nā hopohopo ma Vitro Vitro Vilttoxicity i hōʻikeʻia me nā lole kala kālā10,11. Eia naʻe, i loko o Vitro Cytototicicityʻaʻole i unuhiʻia i loko o Vivo Toxicosity, a ua hōʻikeʻia nā lole AG1 + a me nā lole AG1 + a me nā lole AG1 + a me nā lole AG1 +
Eia, ua noiʻiʻia mākou i ka hopena o ke kaʻa kaʻa kaʻa e pili ana i nāʻano kālā kālā e pili ana i ka biofilm fondro a me vivo. Eia kekahi,ʻo nā hopena o kēia mau lole i nā pane i ka nui a me ka ho'ōla kūʻokoʻa o ka maʻi maʻi.
Ua hoʻohanaʻia nā lole āpau i hoʻohanaʻia. ʻO 3m Kerracent Gel Fel FEBE (3M, Knitsford, UK) He Kīpī i hoʻohanaʻia ma ke kapaʻana i hoʻohanaʻia ma kēia aʻoʻana. ʻEkolu mau kālā kālā kālepa CMC i loiloiʻia,ʻo ia hoʻiʻo 3m Kerracen Agg (3m, Knitsford, UK),ʻo ia kahi 1.7 WT%. ʻO ka paʻakai kālā kālā oxygeated (AG7NO11) i ke kiʻekiʻe o nā kālā kālā kālā (AG1 + AG2 + a AG3 +). I keʻano o ka AG7N11, AG1 +, AG2 + AG3 + AG3 + AG3 + AG3 + AG3 + AG3 + AG3 + AG3 + AG3 + AG3 + AG3 + AG3 + AG3 + AG3 + AG3 + AG3 + AG3 + AG3 + AG3 + AG3 + AG3 + AG3 + AG3 + AG3 + AG3 + AG3 + AG3 + AG3 + AG3 + AG3. ʻO Aquacll Aquacll Aqua Keʻaʻaʻahu e pili ana i ka chloride kālā 1.2% (AG1 +)
Ua hoʻohanaʻia nā mea hana ma kēia noiʻi he Pseudomonsas Aeriginosa NCTC 10781 Health Eriland
Ua ulu nuiʻo Baictia i ka pō ma Muller-Hinton Bolth ('oxoid, Altrisincham, UK). Ua hōʻemiʻia ka moʻomeheu nui i ka pō 1: 100 i nā papaʻo Mueller-Hinton a me 200 μTstone ). )ʻO ka hoʻokumuʻana i ka biofilm biofilm ma 37 ° C no 24 mau hola. Ua ho'āʻoʻia kēia mau chalil biofililms no ka shlingem shackhige.
Eʻokiʻoki i ke kapa lole i loko o 3 cm2 square square a me ka pre-moisten me ka wai deionized. E kau i ka bandage ma luna o ka biofilm o ke kolny ma luna o ka pā kula. Ua hoʻoneʻeʻia kēlā me kēia 24 HA o BioFilm, a me keʻano o BioFteria i loko o ka biofilm (CFU / ML) i ka Dirial Dail Ma hope o 24 mau hola o ka hoʻopukapuka ma 37 ° C, ua hanaʻia nā helu papa pākaukau ma luna o Mulleler-Hinton Agar Plates. Hoʻokomoʻia kēlā me kēia hana a me ka manawa me ka truplicate, a ua hana houʻia nā helu papa no kēlā me kēia hoʻohemo.
Loaʻa kaʻiliʻo Pork Bolly mai nā pua keʻokeʻo nui i loko o 15 mau minuke o ka lukuʻana e like me nā kūlana oʻEulopa. Uaʻokiʻia kaʻili a hoʻomaʻemaʻeʻia me nā holoi kino, a laila brozen ma -80 c no 24 mau hola e hōʻoluʻolu ai i kaʻili. Ma hope o ka holoiʻana, 1 cm2 mau'āpanaʻili i hoʻopauʻiaʻekolu mau manawa, 0.6% sodium hypochlortite, a 70% ethanol no 20 mau minuke i kēlā me kēia manawa. Ma mua o ka weheʻana i ka Epidermis, e wehe i nā mea e noho ana ma ka holoiʻana ma ka holoiʻana i 3 mau manawa i nā pbs sterile pbs. Ua hoʻohālikelikeʻia kaʻili i loko o kahi pā 6-maikaʻi me kahi pahu he 0.45-μn-μ 'Aʻole. ʻO Medium (Dulbecco i hoʻololiʻia e hoʻohuiʻia edit edium - aldrich ltd.).
Ua ulu nāʻoihana kolikaʻo Collonical i like me ka mea i hōʻikeʻia no ka mea aʻo Biofilm Exporsures. Ma hope o ka ho'ōlaʻana i ka biofilm ma ka membrane no 72 mau hola, ua hoʻopili ka biofilm i kaʻili o kaʻili a ua laweʻia ka membrane. Ua hoʻokomoʻia ka biofilm ma luna o ka dermis puaʻa no kahi hola 24 hou ma 37 ° C eʻae i ka biofilm a me ka piliʻana i kaʻili o ka puaʻa. Ma hope o ka loaʻaʻana o ka biofilm a hoʻopiliʻia, a 1.5 cm2 chunging, pre-misiled me kaʻili o kaʻili a iʻole no nā hola he 37 °. Uaʻikeʻiaʻo Bucteria i kaʻikeʻana e ka piliʻana i ka hoʻohanaʻana i ka cetent cell wortoplies E hoʻohana i ka leca dfc425 digital camp45 Ua heluʻia nā wahiʻulaʻula i ka hoʻohanaʻana i ka polokalamu polokalamu PROCIATER 10 (media Cybernetics Inc, Rockville, MD Image-Pro (Mediacy.com). Ua hanaʻiaʻo Scanning Electron Microscopy e like me ka mea i hōʻikeʻia ma lalo nei.
Ua ulu nuiʻo Bacteria i ka nui o ka pō 1: 100 i loko o ka multler-hinton bront. ʻO 200 μL o ka moʻomeheu i hoʻohuiʻia e sterile 0.2 μm whatman golcrane membrane (ʻo Whatman, UK) a pāʻia ma Miller. Ua hōʻikeʻia nā papa biofilm ma 37 ° C no 72 mau hola eʻae ai i ka hana Biofilm.
Ma hope o 3 mau lā o Biofilm Maturation, ua kau pololeiʻia kahi hana 3 cm2 square square ma ka biofilm a incubated ma 37 ° C no 27 ° C no 24 ° C no 24 ° C no 24 ° C no 24 ° C no 24 ° C no 24 ° C no 24 ° C no 24 ° C no 24 ° C no 24 ° C no 24 ° C no 24 ° C no nā hola 24. Ma hope o ka weheʻana i ka bandage mai ka papa biofilm, 1 ml o ka cever cell cetant (invitrgen, ma kaʻaoʻao o kēlā me kēia biofilm no 20 kekona. Ua lukuʻia nā papa ma mua o ka hoʻohanaʻana i nā hoʻololi kala me ka hoʻohanaʻana i kahi kāmela d2300 digital D2300 UK Ltd., Aliʻi UK.
Mākaukau i nā moʻomeheu nui ma Mueller-Hinton Agart, e hoʻololi i nā kolone kanaka i ka 10 ML Multon Bolth ma 37 ° C (100 RPM). Ma hope o ka hoʻonuiʻana i ka uluʻana, ua hōʻemiʻia ka moʻomeheu 1: 100 i loko o ka croller-hinton birst . ^. . ^. Ua hoʻopiliʻia ka biofilm ma kahi e like me ka mea i hōʻikeʻia ma lalo nei.
Ua laweʻia nā hana a pau me nā holoholona ma ke Kulanui o Manchester ma lalo o kahi laikini hana ma lalo o ke keʻena ma lalo o ka hale kūʻai ma lalo o ka hale kūʻai. Nā mea kākau a pau i pili i nā alakaʻi alakaʻi. 'Ewalukūmākahi mau pule-wiki-old -bl / 6jl (envigo,'oxon, uk) i hoʻohanaʻia no nā haʻawina vivo. ʻO nā MID i aesthetized me isoflurane (Piral Scival Credical Care Ltd, West Drayton, UK) a hoʻomaʻemaʻe i kā lākou mau papa dorsal. Hāʻawiʻia kēlā me kēia kiʻi i kahi 2 × 6 mm exmit i loaʻa i ka loaʻaʻana o kahi stifiel biopor bili No nāʻeha biofilm-indorlmm, e kau i ka 72-hola colinnial biofilm e like me ka mea i hōʻikeʻia ma ka hōʻehaʻana ma hope o ka hōʻeha. ʻO hoʻokahi keena kuhina nui o ka lole o ka lole,ʻo ia ka wai me ka wai sterile e mālama i kahi wahi i hōʻehaʻia. Ua hoʻopili pololeiʻia nā lole i kēlā me kēia hōʻeha a ua uhiʻia me 3m Tegaderm Film (3m, Bracknell, Ferndisol a me ka Ferndisol. Buprenorphine (friendcare Cull miceʻekolu mau lā ma hope o ka pōʻino e hoʻohana ana i ka papa inoa 1 a wehe, e hoʻolei i kahi wahi i pono ai.
Hematoxylin (thermofisher scientific) a me ka eosin Ua heluʻia ka wahi Bound a me Reepiithalelization ma ka hoʻohanaʻana i ka polokalamu polokalamu PROFFFRAMT 10 (media Cybernetics Inc, Rockville, MD).
Ua hōʻoleʻia nā'āpana Tissue ma Xylene (Thermofisher Science Science Science Criesty, Loko), UK Ua hanaʻiaʻo immunohistochemistry me ka hoʻohanaʻana i ka Vectastain Elite ABC PK-6104 Kibostay, Burlingname, CA) e like me ka protocol o ka mea hana. ʻO nā antibodies mua i nā neutrophis nimp-r14 (thermofisherʻepekema Abc a me ka vector novaʻulaʻula ulaula Ua loaʻa nā kiʻi i ka hoʻohanaʻana i kahi olympus bx43 microscope a me kahi olymps dp73
Ua hoʻopaʻaʻia nā hiʻohiʻonaʻili i ka 2.5% GlutaReldehyde a me 4% no 4% no ka 0.1 m hepe (pH 7 mau hola ma 4 ° C. ʻO nā hiʻohiʻona i hanaʻia e hoʻohana ana i ka athanol a maloʻo i ka co2 e hoʻohana ana i kahi papa hana strol-bulladidium. Ua hanaʻia nā kiʻi kiʻi me ka hoʻohanaʻana i kahi helu FEI SEI TIMSA 250 SCANING CHICHTCOPE (Thermofisher Scientific) eʻike i keʻano waena o ka hōʻeha.
Ua hoʻopiliʻiaʻo Toto-1 Idide (2 μM) i ka papa kiʻi kiʻi kiʻiʻia a me ka hōʻikeʻana i ka Spienfister Scient). 15-minuke Z-Stack i hanaʻia e hoʻohanaʻia me ka hoʻohanaʻana i kahi TC CP8.
Ua hoʻopaʻaʻia nāʻikepili a me nāʻikepili i kaʻikepili a me ka loiloi i ka hoʻohanaʻana i ka prisppad prism v9 Softkud. ʻO ka nānāʻana o hoʻokahiʻano o keʻano like me nā hoʻohālikelike lehulehu e hoʻohana ana i ka hōʻikeʻana o Dunnet e hōʻike i nāʻokoʻa Ua manaʻoʻia he kumukūʻai p waiwai <0.05 i manaʻo nuiʻia.
Ua loiloi muaʻia ka hana o nā pūnāwai momona o ke kālā kālā ma biofilm o biofilm o saphylococcus a me nā pseudomonsa ma Vitro. Hoʻokomoʻia nā lāʻau kālā kālā i nāʻano kālā likeʻole: Nā lāʻau kālā kālā kālā e hana i nā lāʻau lapaʻau e hana ai a AG1 + ʻO nā lāʻauʻani kala, hiki ke paʻi i ka maʻi ag1 + i like me ka hoʻohuiʻana i ka BATIE / BC, hiki ke hoʻopau i ka biofilm o ka lāʻau antibaakp i kapaʻia he kumu kūʻai. ʻO nā lole a me nā lole i loaʻa i nā clogs overningicated e hana i nā Ag1 + ag2 + a me Ag3 +. Ua hoʻohālikelikeʻia konaʻano me ka lole hoʻokele a me keʻano o ke kapa komoʻana mai nā fibers gelled. Ke nānā nei i ke koena o Blifteria i loko o ka biofilm i loiloi i kēlā me kēia 24 mau hola no 8 mau lā (kiʻi 1). I ka lā 5, ua hoʻoponopono houʻia ka biofilm me 3.85 × 105s. Saaphylococcus aureus a iʻole 1.22 × 105p. Ausuginosa e loiloi i ka hoʻihoʻi biofilm. Hoʻohālikelike i nā lole hoʻokele waiwaiʻoleʻole, ag1 + mau lole i ka hopena liʻiliʻi ma ka maʻamau ma luna o nā haleʻo Staphylococus ma luna o 5 mau lā. I ka hoʻohālikelikeʻana, e nāʻaʻahu i loaʻa i ka OxyGenGated Ag a me Ag1 + + EdAT / BC Salts i loko o ka Biofilm i loko o 5 mau lā. Ma hope o ka hana houʻana me ka hoʻolālā planktotic i ka lā 5,ʻaʻohe mea hou o ka biofilm i nānāʻia (Fig. 1).
ʻO ka heluʻana o ka baict bucteria ma Staphylococcus AUureus a me Pseudomontas Aeriginos Aeriginos Aeriginos Aeriginos Aeriginos ma hope o ka mālamaʻana i nā kolepa. Ua mālamaʻia nā ciafilm chanifilom o Staphylococcus Aireus a me Pseudomontas adiurinos Ma hope o 5 mau lā, ua hoʻoponopono houʻia ka biofilm me 3.85 × 105 mau. Saaphylococcus aureus a iʻole 1.22 × 105p. ʻO nā colinies o ka bacterioplanconton pseudomontas aeriginos Aeriginoas i hanaʻia e loiloi i ka hoʻihoʻi biofilm. Hōʻike nā kiʻi kiʻi i nā hōʻike +/- maʻamau.
E hōʻike i ka hopena o nā lole kālā kālā ma biofilm biofilm, ua hoʻopiliʻia nā lole biofilms i nāʻili porcine ex vivo. Ma hope o 24 mau hola, laweʻia ke kapa lole a ua paʻa ka biofilm me kahi mea maikaʻi loa,ʻo ia ka metabolilized e kahiʻulaʻula. Ua mālamaʻia nā biofilms me nā lole hoʻomalu, e hōʻike ana i ka heleʻana o ka biofia i loko o ka biofilm biofilm (kiʻi 2a). I ka hoʻohālikelikeʻana, ua mālamaʻia ka biofilm me nā anianiʻo Ag mau megyals Ua nānāʻia ka polū polū a me nā kalaʻulaʻula i loko o nā biofilms i mālamaʻia me nā lole o AG1 + -Canting i loko o ka biofilm e hōʻike ana i nā wahiʻaʻole i hoʻopilikiaʻia e ke kapa kālā kālā (kiʻi 2D). ʻO ka heluʻana o ka ikaika Ua hana likeʻo Ag1 + + + edta / bc i nā lole hoʻonaninani a me ka oxyngated agyisated, me nā kumukūʻai ola o 13% a me 14%, e pili ana. Ua hoʻemiʻia hoʻi ka AG1 + Eʻaʻahu i ke aniani barmerer ma o 21%. Ua nānāʻia kēia mau biofilms i ka hoʻohanaʻana i nā kiʻi uila uila (sem). Ma hope o ka mālamaʻana me ke kāhikoʻana i ka lole a me ka lole o Ag1 + e hoʻohana ana i ka Msedomonas Aerice Hiki ke manaʻoʻia nā file collagen e like me ka hana o kaʻili o kaʻili puaʻa puaʻa (kiʻi 2g). Ma hope o ka mālamaʻana me AG1 + + Edta / BC eʻaʻahu ana, i nā plaques a me nā mea i hoʻopaʻaʻia ma lalo o nā papa file collage.
ʻO ka'ikeʻikepili o Psedomontas Aeriginosasa biofilm ma hope o ka mālamaʻana i ke kala kala kala. (A-D) BACTERER VABILING IN PSEUDOMONAS BIFOFILMS G G G G G G G G G GROULL DYE 8 mau hola ma hope o nā lole kūʻai kūʻaiʻole. ʻO nā pēpē olaʻo Baicteria he pink (E)ʻO ka heluʻana o Pseudomonas Aeriginosa biofilms i nā kiʻiʻoniʻoni a me ka lole a me ke kapaʻole ma keʻano o ke kala. Sem sub star = 5 μm. (J-m) Biofilms colifilms great grew i nā kānana a ua hoʻopaʻaʻia me ka nui o ka prettoblue
No ka hoʻoholoʻana o ke kelepona kokoke i waena o nā Kokuahi a me nā BiofililS i hoʻokūkūʻia e ka pono o nā keʻena. ʻO Biofilm Biofilm iʻeleʻeleʻeleʻeleʻeleʻele i ke kala (kiʻi 2J). I ka hoʻohālikelikeʻana i nā biofilms i mālamaʻia me nā lole e loaʻa ana i nā calts oxygen agygent (a iʻole 2 Hōʻike kēia mau kalaʻulaʻula i ka heleʻana o keʻano o ka bucteria a pili pū me ka mea e pili ana i ka wahi o ke kapa. ʻO kēia mau mea e hana ai i nā wahi make i nā wahi make eʻae i ka bacteria i loko o ka biofilm biofilm e ola ai.
E loiloi i ka hana o nā lole kālā kālā ma Vivo, ua hoʻonuiʻia nā mea paʻaʻole i nā lole o ka MIce S. AeriginoSa i nā lole nonditumichms Ma hope o 3 mau lā o ka mālamaʻana, ua hōʻikeʻia ka loiloi kiʻi kiʻi maxrosscop i hōʻikeʻia i ka liʻiliʻi o nā lole paʻakai uila E hōʻoia i kēia mau nānāʻana, ua hōʻiliʻiliʻia nā hōʻeha a me nā mea hōʻeha a me ka reepithelilization a me nā mea hou e hoʻohana ai i ka heosatoxy a me nā mea e pili ana i ke kiʻi
ʻO ka hopena o nā lole kālā ma keʻano hōʻeha a me ka hoʻoponopono houʻana o nā hōʻeha i hōʻehaʻia me nā biofilms. (A-H) i nā mea liʻiliʻi liʻiliʻi e hoʻopiliʻia me nā biofilms o Pseudomonas Aeriginosa (E-D) ʻaʻahu. Nā kiʻi macrosscopic. Nā hōʻeha o nāʻili me ka AG1 + + EDTA / BCʻaʻahu. (IL) Luna Nui Pseudomonas Aeriginos i ka maʻi Aeriginosa ʻO ka heluʻana o nā wahi hōʻeha (M, O) a me ka hapanui o ka reepithelitalization (n, p) o nā mea hōʻeha Hōʻike nā kiʻi kiʻi i nā hōʻike +/- maʻamau. * Ka mea 'p = <0.05 ** e loaʻa ka P = <0.01; MacrossCopic Scale = 2.5 mm, thistalogical scale = 500 μm.
ʻO ka heluʻana o nā wahi hōʻeha i nā hōʻeha i loaʻa me Pseudomontas Aeriginos Aferunos i hōʻikeʻia i ka nui o nā Aggyalts ʻoiaʻiʻo. hiki i kahi kūlana koʻikoʻi (kiʻi 3M). P = 0.423). Ua mālamaʻia nā hōʻeha me AG1 + a iʻole AG1 + + EDTA / BC Ka mālamaʻana me ka oxygenated oxygancated ag speet i hāpai houʻia i kahi epithelianization e hoʻopiʻi ai ma mua o ka lole o ka Actimicrobial. Kiʻi 3n). . ^. , a).
Like like like me nā wahiʻeha a me ka regeneral regeneration regeneration i nā hōʻeha i kaʻeha me S. Aureus biofilms (kiʻi 3o). ʻO nā lole i loaʻa i nā waiwai kālā o OXYGEND i hāʻawiʻia (2.0 MM2) e 23% i hoʻohālikelikeʻia me ka hōʻemiʻana (P = 0.304). 3. ʻO ka hoʻohuiʻiaʻana o ka wahi kīnā ma ka AG1 + Pūnaewele Hoʻolālā (2.4 MM2) ʻO ka momona o ka oxygen o ka Ag e hoʻolaha hou i nā hōʻeha hou o S. Aureus Biofilm (12%, P = 0.003) (Kiʻi 3P). AG1 + KAKAU
E hōʻike i ka hopena o nā keʻena kālā ma ka Biofilm Biofilm, toto 1 a me Syto he 60 o nā kiʻi. 4). ʻO Toto 1 Iodide he cell-impermeable dye hiki ke hoʻohanaʻia eʻike pono i nā maʻi nucleclush. ʻO Syto 60 ka mea i hoʻohanaʻiaʻo ia i hoʻohanaʻia e like me ka mea hoʻohālikelike. ʻO ka nānāʻana o Toto 1 a me Syto 60 idide i nā biofildas aeruudomonas Aerice ka mea i loaʻa i nā salts oxyget ag a me AG1 + +DA / BC. ʻO AG1 + eʻaʻa me kaʻole o nā mea hoʻohui antibiofilm i hōʻemi nuiʻia i nā mea i hoʻopiliʻia me Pseudomontas Aerice.
Ma Vivo e loaʻa ana i ka Biofilm Biofilm ma hope o 3 mau lā o ka mālamaʻana me ka hoʻokele a me ke kapa a me ke kala paha. ʻO nā kiʻi Confolom o Psedomonsas Aeriginosa E nānā i nā maʻi nucleclular nucleclular, e hoʻohana i ka syto 60 (ʻulaʻula). Acpids. P. scanning electron microscopy o nā hōʻeha i ka pseudomonas Aeriginosa (Eeruginosas Aerical (M-P) Sem sub star = 5 μm. ʻO ka noʻonoʻoʻana i ka pahu scale bar = 50 μm.
Ua hōʻikeʻia ka Microscopy Electron Microscopy i nā Mili i hoʻopaʻaʻia me Biofilm o Pseudomonas Aeruginos
No ka loiloiʻana i ka hopena o nā lole kālā ma ka hōʻehaʻana i nā momi biofilm-indhelom Ka manaʻo nui o nā neutrophils a me nā macrophages i loko. ʻO ka Tissulation Tissulation. Kiʻi 5). Ua hōʻemi nā lole kālā a pau i ka helu o nā neutrophils a me nā macrophages i nā mea hōʻeha i hoʻohālikelikeʻia me nā lole adiuricosa Eia nō naʻe, mālamaʻia me ke kapa o ka paʻakai o ka paʻakai o ka momona ʻOiaiʻo AG1 + + EDTA / BC he hopena nui loa i ka biofilm, a hoʻemiʻia nā pae macrophil ma mua o ka meaʻoi aku ka nui o ka AG1 +. Ua nānāʻia nā meaʻeha a me nā meaʻeha a S. Aureus Biofilm i ka lole me ka ag (P = <0.00013). Nānāʻia nāʻano likeʻole no neutropenia. bandage (Fig. 5k). Eia nō naʻe,ʻo ia wale nō ke kapa o ka OXYGANEST OFTER i hōʻikeʻia i kahi hōʻemi nui ma ka helu o nā macrophages i hoʻohālikelikeʻia me S. 0,039).
Ua heluʻiaʻo Neutrophals a me nā Macrophaphages i nā hōʻeha me nā Pseudomonsas Aeriginoshua a me Staphylococcus a me nā mea kūʻai aku a me nā lāʻau lapaʻau. Neutrophils (ad) a me nā macrophages ʻO ka heluʻana o nā neutrophils (i a me k) a me nā macrophages N = 12 i kēlā me kēia hui. Hōʻike nā kiʻi i nā kiʻi +/- maʻamau maʻamau ***ʻo ka P = <0.001; Hōʻikeʻo P = <0.0001).
A laila loiloi mākou i ka hopena o nā lole kālā ma ke ola kūʻokoʻa. Ua mālamaʻia nā hōʻeha o nā mea i hanaʻoleʻia me ke kapa komo a me ke kapa kalaʻole a iʻole ke kapa kala no 3 mau lā (kiʻi 6). Ma waena o nā lole kālā i ho'āʻoʻia, ua mālamaʻia nāʻeha a me nā hōʻeha o ka paʻakai o ka paʻakai ʻO ka heluʻana o ka wahi hōʻeha e hoʻohana ana i ka loiloi o ka huiʻana ma hope o ka mālamaʻana i ka papa hana o ke kumukūʻai (P = 0.48) (FIE .. 6i). Hoʻohālikelike,ʻaʻohe hoʻemiʻana i ka wahi hōʻeha i mālamaʻia ma hope o ka mālamaʻana me AG1 + (28 mm2, p = 0757) a iʻole ag2, p = 0757) a iʻole ag2 Uaʻikeʻia ka hoʻopaʻiʻana o Epiellital me ka The Ag o Ag Gineysol i hoʻohālikelikeʻia i ka hui mana (30% ʻO ke kapa me nā mea hanaʻo Janysols e hoʻolaha hou i ka Epithelialization. -Epitlalization o nāʻehaʻoleʻole i loaʻaʻoleʻia. I ka hoʻohālikelikeʻana, mālamaʻia me AG1 + a iʻole AG1 + + Esta / BC i ka hopena i hoʻohālikelikeʻia.
Ka hopena o ke kāhiko kālā kālā e hōʻeha ai i ka hōʻehaʻana i nā moice iʻole me ka ukuʻole. (Ad) nā kiʻi macroscopic i nā hōʻeha ma hope oʻekolu mau lā o ka mālamaʻana me ka lole kūʻai a me ke kapa kala. (Eh) Nā hōʻailona e hōʻikeʻia ai nā'āpana hōʻeha i hoʻopaʻaʻia me ka hematoxylin a me Eosin. Ka heluʻana o ka wahi hōʻeha (i) a me ka pakeneka o ka reepithellutionalization (J) i heluʻia i ka māhele o ka hōʻike kiʻi Hōʻike nā kiʻi kiʻi i nā hōʻike +/- maʻamau. *ʻO keʻanoʻo P = <0.05.
Loaʻa i ka mōʻaukala lōʻihi o ka hoʻohanaʻana i ka hana antimicrobial i ka hōʻehaʻana, akā he nui nāʻano hana likeʻole a me nāʻano hana likeʻole i nāʻokoʻa antiimicrobial 18. Eia kekahi,ʻo ka antibiofilm eʻike i nā'ōnaehana kālā kālā kūikawāʻaʻole maopopo. ʻOiaiʻo ka paneʻana o ka Host Immune e pono ai e kū'ē i ka Bigytonic Bigteria ʻO Plakktonic Baicteria e hui pūʻia e Phagogytod i nā macrophages, akā i loko o nā poki a me ka hoʻokuʻuʻana i ka hoʻolahaʻana i ka hoʻolaha Ua nānāʻiaʻo ia e hiki i kekahi lekoicytes ke komo i ka biofilms21 akā hikiʻole ke hiki ke hoʻonui i ka pale Pono e hoʻohanaʻia kahi ala hoticistic e kākoʻo i ka pane o ka hale hoʻokipa e kū'ē iā BioFilm. Hiki i ke kīwīʻeha ke hōʻeha i ka biofilm a wehe i ka hapanui o ka bioburden No laila, hiki i nā mea lapaʻau antimicrobial e like me nā mea kūʻai kālā e hiki ke kākoʻo i ka pane o ka ADMUNE ACT i ka hopena biofilm. Ka hoʻohālikelikeʻana, kaʻikeʻana, he maʻi kūpono, a me ka hāʻawiʻana i ka moku e hōʻike i keʻano antiimicrobialness o ke kālā. I nā makahiki i hala iho nei, hoʻomaʻamaʻa i nāʻoihana kālepa kālā i hana hou i kēia mau lāʻau lapaʻau hou aʻe. E like me keʻano o nā loea kālepa kālā, he mea nui ia e hoʻomaopopo i ka pono o kēia mau keʻena i kāohi i ka loaʻaʻole o ke kālā ma keʻano a me ka ho'ōla.
Ma kēia noiʻi, e hoʻohālikelike mākou i ka hopena o nā lole kālāʻelua e pili ana i nā kapa kālā kālā e pili ana i ka OG1 + i nā hiʻohiʻona Vivo. Ua loiloi pū mākou i ka hopena o kēia mau lole i keʻano hōʻeha a me ke ola kūʻokoʻa. E hōʻemi i ka mana o ka hāʻawiʻana i ka Matrix lawe, ua ho'āʻo nā mea kūʻai kālā a pau i hōʻikeʻia i ka carboxymelfulfulfulloselcellselllselllfulfulloselcelselcelselcellselllsellselllsellselllfulfulloselcelselcelselcellselfulfulloselcelselcelselcellselfulloselcelselcellsellsellselllsellsellselllselllfulfulloselcellselfullose.
ʻO kā mākou loiloi uku mua o kēia mau kolepa kālā e kū'ē i nā pomana a pseudomonos,ʻaʻole pono e pepehi i nā mea kūʻai kālā biofilm i loko I kekahi mau lā. Ma kahi o kēia mau lole e pale ai i ka hana houʻana o biofilm ma ka hoʻolahaʻana i nā bracttotic placteria. ʻO ka AG1 + Eʻaʻahu i ka chlordide chlordide colloride,ʻo ka pūpū kālā likeʻole a me ke kumu kūʻai kālā i loko o ka biofilm ma luna o ka manawa like. ʻO ka nānāʻana he AG1 + + HDA / BC e pili ana i ka biofilm ma mua o kahiʻano o ka biofilm chlofilm wahi'ē aʻe15. Kākoʻo kēia mau hopena i ka manaʻoʻo BC a me Edta e pāʻani i kahi hana hou e hāʻawi ana i ka hana kūponoʻole i ka hanaʻana i ka nui o ka nui. Uaʻike mākou i kēlā me ka oxygeated oxyganceg e hana ana i ka AG2 + A me AG3 +O. Eia nō naʻe, ma muli o ke kiʻekiʻe o Redox Eia kekahi, he nui nā lole likeʻole e hana i ka AG1 + ions i ho'āʻoʻoleʻia ma kēia aʻoʻana. ʻO kēia mau lole i hoʻomohalaʻia no nā pupu kalaʻole, nāʻinoʻino, a me nā papani, kahi e hoʻopiʻi ai i ke kumu o nā biofilms. ʻO ia nō naʻe e pono ana heʻokoʻa heʻokoʻa nāʻano'ē aʻe a me nā model Vivo i hoʻohanaʻia e loiloi i ka hopena o nā kumuwaiwai e kū'ē i ka hana hōʻeha. ʻO keʻano o ka hiʻohiʻona i hoʻohanaʻia, a me ka paʻakai o ka paʻakai a me kaʻike o ka mīkini i hoʻohanaʻia ma kēia mau hoʻohālike, e hoʻohālikelike i ka pono o ke kapa. I loko o kā mākou hana vivo, uaʻae mākou i ka biofilm i ka maikaʻi i ka vitro a laila e hoʻoneʻeʻia i keʻano dermal o kaʻeha. ʻO ka paneʻana o ka kiʻi kiʻi kiʻi kiʻi e pili ana i ka blacttonic i hoʻopiliʻia i ka biorteria ʻO ka hoʻohuiʻana o Biofilm i kahi hōʻeha hōʻeha i ka hopena o ka Acmulm o ka biofilm e hoʻokumu i ka hōʻeha ma waena o ka ho'ōlaʻana No laila, hiki iā mākou ke hoʻololi i ko mākou mea e loiloi iā mākou e loiloi i ka maikaʻi o nā lole antimicrobial ma mua o ka hoʻomakaʻana o nā hōʻeha.
ʻIke pū mākou i keʻano lole e pili ana i keʻano o ka hana o nā lole kālā ma Vitro-Grown Biofilms a me kaʻili porcine. Hoʻopili i ka hoʻopiliʻana me kaʻeha i manaʻoʻia he koʻikoʻi no keʻano antimicropial o ka lole o ka lole24,25. ʻO nā lole e loaʻa ana i nā slats oxygen agyged i hoʻopili pūʻia me nā biofilms I ka hoʻohālikelikeʻana, i ka manawa e mālamaʻia me AG1 + a me AG1 + + EDTA / BC me nā mea waiwai nui. ʻO kēia mau kapa komo i nā mea nui ma ka lōʻihi o ka lōʻihi o ke kapa, e hana ana i nā wahi i hanaʻia e pale aku ai me ka biofilm. I kā mākou mau noiʻi vitro, nā wahi piliʻole i paleʻia i ka pepehiʻana o ka biofia i loko o ka biofilm biofilm. Hoʻoliloʻia mākou i ka waiwai bacterial wale nō ma hope o 24 mau hola o ka mālama; ʻO ka manawa, e like me ka mea e hoʻonani ai i ka lole, he mau mea make paha, e hōʻemi paha i ka wahi o kēia wahi no kēia mau bula nui. Eia naʻe, hōʻikeʻike kēia i ka nui o ka hoʻohuiʻana o ke kapa,ʻaʻole wale keʻano kālā i keʻaʻahu.
ʻOiai e kūpono ana nā haʻawina Views no ka hoʻohālikelikeʻana i ka hopena o nāʻenehana kala likeʻole, he mea nui hoʻi kaʻike i nā hopena o ke kumu hao ma nā kumupepila. ʻO ka hopena o kēia mau lole i nānāʻia i nā biofilms i nānāʻia e hoʻohana ana i ka microscopy a me nā neʻe o ka biofilc e hoʻohana ana i ka biofilt a me extracellular. Uaʻike mākou ma hope o 3 mau lā o ka mālamaʻana, he pono nā lole a pau i ka hoʻemiʻana i nā hōʻeha bifilmom Ua hōʻike pūʻiaʻo Scanning Electron Microscopy i nā mea liʻiliʻi loa i hōʻikeʻia i nā hōʻeha a me ke kapa o AG1 +. Hōʻike kēia mauʻikepili i ka lole kala i ho'āʻoʻia e like me nā pae aʻoʻo Bifilm, ke kākoʻoʻana i ka pono kīhini mauʻo Bifilm ka hoʻohanaʻana i nā kiʻi kala kālā. ʻO ka mālamaʻia ma mua o ka hana kino kino e wehe i ka hapanui o ka biofilm.
ʻO nā mea hōʻeha pinepine i kahi kūlana o keʻano nui, me ka nui o nā cell i ka wā e pono ai ke kauʻana i ka maʻi Ke hoʻonui neiʻo Biofilms i kēiaʻano hōʻehaʻeha e pili ana i ka ho'ōlaʻana i nāʻano o nāʻano propite27. E like me nā lole kālā e lilo ai i mea nui, he mea nui ia e hoʻomaopopo i ka hopena i loaʻa iā lākou ma keʻano o ka hōʻeha a me ka ho'ōla.
Hoihoi,ʻoiai nā lole kālā a pau i pili i ka hui biofilm Ke kākoʻo nei kēia mauʻikepili i kā mākou hana mua17 a me nā mea o Kaalan et al. (2017) 28, e hōʻike ana i ka palekana palekana a me nā hua'ōlelo kālā o oxygen mau loiloi o nā kala kala.
ʻO kā mākou aʻoʻana i kēia manawa i nāʻokoʻa i kaʻenehana kālā ma waena o nā lole kālā antiimicrobial a me ka hopena o kēiaʻenehana a me ke ola kūʻokoʻa. Eia nō naʻe,ʻokoʻa kēia mau hopena i nā haʻawina mua e hōʻike anaʻo AG1 + + HDA / BC i ka hoʻohanaʻana i nā'āpana hoʻomaha o ka blivo. Eia nō naʻe,ʻo kēia paha ke kūpono paha i nāʻokoʻa i nā hiʻohiʻona holoholona, nā manawa e hoʻopaʻaʻia ai, a me nāʻano noi noi bacter29. I kēia hihia, ua hōʻehaʻia nā paʻakikī'ōpala 12 nā lā ma hope o ka hōʻehaʻana eʻae i ka biofilm i hoʻokahi manawa. Ua kākoʻoʻia kēia e kahi haʻawina i hōʻikeʻia i nā wāwae i hoʻopaʻaʻia i nā ulcers i hanaʻia me ka 3 mau pule hou o AG1 + + EDTA ka hoʻohanaʻana i nā meaʻole-antimicrobial. nā lāʻau lapaʻau. Nā lole cmc e hōʻemi i ka nui o nā ulcers30.
ʻAʻole i hōʻikeʻia kekahi mauʻano a me nā koina o ke kala ma ke cytotopic ma Vitro 11, akāʻaʻole e unuhi hou kēia mau hopena i nā hopena maikaʻiʻole i loko o Vivo. Eia kekahi, holomua ma kaʻenehana kālā a me kahiʻike maikaʻi loa i nā mea kālā kālā a me nāʻike i nā lole kālā he nui. Eia nō naʻe, e like me keʻano o kaʻenehana kālepa kālā, he mea nui ia e hoʻomaopopo i ka hopena o kēia mau lāʻau lapaʻau ma ka hōʻeha kai .a33. Ua hōʻikeʻia ma mua e hoʻonuiʻia ka nui o ka nui o ka helu houʻana i ka hoʻonuiʻiaʻana o nā macrophators anti-inflamortory m1 phenotype. Uaʻikeʻia kēia ma kahiʻano mouse mua ma kahi e hoʻohālikelike ai nā lāʻau lapaʻau caldrogel
Hiki ke hōʻike i nā mea hōʻeha o nā meaʻehaʻeha a ua nānāʻiaʻo ia e nānā i mua o ka heleʻana o nā neutophil Ma kahi aʻo ma Neutrophil-deplephil i hanaʻia, ke heleʻana o ka heleʻana o nā neutrophils i ka repithelitalization. Ke alakaʻi nei ka heleʻana o nā neutrophils i nā pae kiʻekiʻe i nā kiʻekiʻe o ka palekana a me nāʻano hana gectroxide, e like me ka nui o ka maʻi a me ka hydrogen peroxide. Pēlā nō hoʻi, he hoʻonui i nā helu macrophage, ināʻaʻole i hoʻopaʻaʻoleʻia, hiki ke alakaʻi i ka hōʻehaʻana i ka hōʻeha. He mea nui loa kēia hoʻonui ināʻaʻole hiki i nā Macrophages ke hoʻololi i kahi Phenotype Pro-inflamortorpe i kahi phesedpe pro-ho'ōla ma kahi o ka ho'ōlaʻoleʻana i ka hopena Ua nānā mākou i kahi hōʻemi i nā neutrophils a me nā macrophages i nā hōʻeha bifilm ʻO kēia hōʻemi paha he hopena kūpono loa o ka paneʻana o ka nui o ka nui, e pane ana i ka bioburden, a iʻole ka hōʻehaʻana i ka bioburden i ka hopena o ka ho'ōlaʻana. ʻO ka hōʻemiʻana i ka helu o nā'āpana o nā wahi i hōʻehaʻia i ka hōʻeha e mālama ai i kahi nohona e hōʻeha ai i ka hoʻomaha. ʻO ka hana o ka hana o ka hana o keʻano o ka ho'ōlaʻana o ka maʻi a me ka hopena o ka ofyson a me nā mea maʻi o ka maʻi agyen.
ʻO nā mea iʻikeʻoleʻia e ho'ōla i nā hōʻeha i hōʻehaʻia i kahi pilikia no nā kaukaʻelua a me nā maʻi. ʻOiai he nui nā lole e noi ana i ka hana antimicrobial, e hoʻopili pinepineʻia nā mea noiʻi i nā mea nui e hoʻonui ai i ka micionenting. Hōʻike kēia noiʻi i nā mea hana likeʻole i loaʻa i ka hopena antimicrobial'ē aʻe a me nā mea nui, he mea nui, nā hopena likeʻole ma keʻano o ka maʻiʻeha. ʻOiaiʻo kēia ma Vitro a me Vivo Hōʻike hōʻike hōʻike i ka hopena o kēia mau lole a me nā hoʻomau i hoʻomaloʻoʻana i ka hopena o kēia mau lole.
Ua hoʻohanaʻia nā Datasts a / a iʻole a i kuhikuhiʻia i ka wā o ke aʻoʻana i kēia manawa mai ka mea kākau e pili ana i ka noi kūpono.
Post Time: Jul-15-2024